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c freundii collection strains atcc 6879  (ATCC)


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    ATCC c freundii collection strains atcc 6879
    C Freundii Collection Strains Atcc 6879, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+freundii+atcc+6879/Citrobacter+freundii/pmc06730498-873-15-19
    Average 90 stars, based on 5 article reviews
    c freundii collection strains atcc 6879 - by Bioz Stars, 2026-09
    90/100 stars

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    Polymerase Chain Reaction:

    Article Title: Characterization ofqnrB-Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: Our attempt to clone the qnrB-like gene of C. freundii ATCC 6879 was unsuccessful. .. PCR amplification and sequencing of the region between pspF and sapA confirmed that C. freundii ATCC 6879 harbors a fulllength qnrB gene (qnrB60) and that C. braakii ATCC 51113T harbors qnrB61. .. QnrB60 in C. freundii ATCC 6879 was found to differ by one amino acid from QnrB38 (A202V).

    Article Title: Characterization of qnrB -Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: .. In C. ... PCR amplification and sequencing of the region between pspF and sapA confirmed that C. freundii ATCC 6879 harbors a full-length qnrB gene ( qnrB60 ) and that C. braakii ATCC 51113 T harbors qnrB61 . .. QnrB60 in C. freundii ATCC 6879 was found to differ by one amino acid from QnrB38 (A202V).

    Article Title: Characterization of qnrB -Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: In the present study, to clarify this linkage more generally and robustly, reference Citrobacter strains of the American Type Culture Collection (ATCC), including type strains, nonclinical isolates, and a strain from the preantibiotic era, were subjected to detection and characterization of the qnrB -like gene ( 9 – 11 ). (This work was presented in part at the 49th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, CA, 2009.) .. Five Citrobacter strains of the ATCC, including three type strains, were subjected to qnr detection by PCR, i.e., Citrobacter freundii ATCC 8090 T , C. freundii ATCC 43864, C. freundii ATCC 6879, Citrobacter braakii ATCC 51113 T , and Citrobacter koseri ATCC 27028 T (see Table S1 in the supplemental material). .. Two strains, namely, C. freundii ATCC 6879 and C. braakii ATCC 51113 T , were positive for qnrB with two different primer pairs. qnrA and qnrS were not amplified in any strain.

    Amplification:

    Article Title: Characterization ofqnrB-Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: Our attempt to clone the qnrB-like gene of C. freundii ATCC 6879 was unsuccessful. .. PCR amplification and sequencing of the region between pspF and sapA confirmed that C. freundii ATCC 6879 harbors a fulllength qnrB gene (qnrB60) and that C. braakii ATCC 51113T harbors qnrB61. .. QnrB60 in C. freundii ATCC 6879 was found to differ by one amino acid from QnrB38 (A202V).

    Article Title: Characterization of qnrB -Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: .. In C. ... PCR amplification and sequencing of the region between pspF and sapA confirmed that C. freundii ATCC 6879 harbors a full-length qnrB gene ( qnrB60 ) and that C. braakii ATCC 51113 T harbors qnrB61 . .. QnrB60 in C. freundii ATCC 6879 was found to differ by one amino acid from QnrB38 (A202V).

    Article Title: Characterization of qnrB -Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: Five Citrobacter strains of the ATCC, including three type strains, were subjected to qnr detection by PCR, i.e., Citrobacter freundii ATCC 8090 T , C. freundii ATCC 43864, C. freundii ATCC 6879, Citrobacter braakii ATCC 51113 T , and Citrobacter koseri ATCC 27028 T (see Table S1 in the supplemental material). .. Two strains, namely, C. freundii ATCC 6879 and C. braakii ATCC 51113 T , were positive for qnrB with two different primer pairs. qnrA and qnrS were not amplified in any strain. .. In order to perform cloning of qnrB , a BamHI-digested genomic DNA fragment containing the qnrB -like gene and BamHI-digested, bacterial alkaline phosphatase (BAP)-treated pUC119 (TaKaRa Bio Inc., Otsu, Japan) were ligated with TaKaRa Ligation Mighty Mix (TaKaRa Bio Inc.) and chemically transformed into Escherichia coli TOP10 (Invitrogen, Carlsbad, CA).

    Article Title: Characterization ofqnrB-Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: Five Citrobacter strains of the ATCC, including three type strains, were subjected to qnr detection by PCR, i.e., Citrobacter freundii ATCC 8090T, C. freundii ATCC 43864, C. freundii ATCC 6879, Citrobacter braakii ATCC 51113T, and Citrobacter koseri ATCC 27028T (see Table S1 in the supplemental material). .. Two strains, namely, C. freundii ATCC 6879 and C. braakii ATCC 51113T, were positive for qnrB with two different primer pairs. qnrA and qnrS were not amplified in any strain. .. In order to perform cloning of qnrB, a BamHI-digested genomic DNA fragment containing the qnrB-like gene and BamHI-digested, bacterial alkaline phosphatase (BAP)-treated pUC119 (TaKaRa Bio Inc., Otsu, Japan) were ligated with TaKaRa Ligation Mighty Mix (TaKaRa Bio Inc.) and chemically transformed into Escherichia coli TOP10 (Invitrogen, Carlsbad, CA).

    Sequencing:

    Article Title: Characterization ofqnrB-Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: Our attempt to clone the qnrB-like gene of C. freundii ATCC 6879 was unsuccessful. .. PCR amplification and sequencing of the region between pspF and sapA confirmed that C. freundii ATCC 6879 harbors a fulllength qnrB gene (qnrB60) and that C. braakii ATCC 51113T harbors qnrB61. .. QnrB60 in C. freundii ATCC 6879 was found to differ by one amino acid from QnrB38 (A202V).

    Article Title: Characterization of qnrB -Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: .. In C. ... PCR amplification and sequencing of the region between pspF and sapA confirmed that C. freundii ATCC 6879 harbors a full-length qnrB gene ( qnrB60 ) and that C. braakii ATCC 51113 T harbors qnrB61 . .. QnrB60 in C. freundii ATCC 6879 was found to differ by one amino acid from QnrB38 (A202V).

    Functional Assay:

    Article Title: Characterization of qnrB -Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: This result was consistent with the presence of an intact SOS box upstream of qnrB61 in C. braakii ATCC 51113 T . fig ft0 fig mode=article f1 Fig 2 caption a4 The change in qnrB61 , recA , and lexA transcription due to fluoroquinolone exposure in C. braakii ATCC 51113 T . After 1 h of incubation, levofloxacin was added at a final concentration of 1× MIC (0.06 μg/ml), 0.5× MIC (0.03 μg/ml), ... As quinolone is a synthetic antimicrobial agent that was introduced into the clinical setting in the early 1960s, the quinolone resistance determinant was not expected to be prevalent in nature before the development and practical use of quinolones. .. Our findings in the present study, however, indicated that there should be a revision of this framework; a “functional” qnrB61 gene was identified on the chromosome of the type strain of C. braakii , ATCC 51113 T , which is a member of the C. freundii complex and was isolated from a snake ( http://www.atcc.org/ ) ( 9 ). qnrB60 was also found in C. freundii ATCC 6879, which was an isolate from milk set for cottage cheese before 1932 in the preantibiotic era, long before the synthesis of quinolones ( 11 ). .. Moreover, ATCC 8090 T , a type strain of C. freundii isolated before 1931, harbored a truncated qnrB pseudogene ( 10 ).

    Isolation:

    Article Title: Characterization of qnrB -Like Genes in Citrobacter Species of the American Type Culture Collection
    Article Snippet: This result was consistent with the presence of an intact SOS box upstream of qnrB61 in C. braakii ATCC 51113 T . fig ft0 fig mode=article f1 Fig 2 caption a4 The change in qnrB61 , recA , and lexA transcription due to fluoroquinolone exposure in C. braakii ATCC 51113 T . After 1 h of incubation, levofloxacin was added at a final concentration of 1× MIC (0.06 μg/ml), 0.5× MIC (0.03 μg/ml), ... As quinolone is a synthetic antimicrobial agent that was introduced into the clinical setting in the early 1960s, the quinolone resistance determinant was not expected to be prevalent in nature before the development and practical use of quinolones. .. Our findings in the present study, however, indicated that there should be a revision of this framework; a “functional” qnrB61 gene was identified on the chromosome of the type strain of C. braakii , ATCC 51113 T , which is a member of the C. freundii complex and was isolated from a snake ( http://www.atcc.org/ ) ( 9 ). qnrB60 was also found in C. freundii ATCC 6879, which was an isolate from milk set for cottage cheese before 1932 in the preantibiotic era, long before the synthesis of quinolones ( 11 ). .. Moreover, ATCC 8090 T , a type strain of C. freundii isolated before 1931, harbored a truncated qnrB pseudogene ( 10 ).



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